Roche GS-FLX (454) Next Generation Sequencing

The Roche Genome Sequencer FLX System (GS-FLX or "454") moves DNA sequencing to a chip based platform dramatically reducing the work and price required for genome level sequencing projects. This is performed by simplifying the up front work required to generate and amplify a genomic library and by massive parallel sequencing in miniature an entire library simultaneously during a single run on the GS-FLX/454 instrument.
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Solexa Next Generation Sequencing

Like the Roche GS-FLX sequencer the Solexa Genome Analyzer (GA) moves DNA sequencing to a chip based platform dramatically reducing the work and price required for genome level sequencing projects. An entire genomic library is amplified for sequencing simultaneously on a chip via bridge-PCR and then each clonally amplified product (cluster on the chip) is sequenced by synthesis on the Solexa GA by massive parallel sequencing across the entire chip (Solexa 'flow cell'). During sequencing each terminating base is labeled with one of four fluorophores allowing for the reading of one base per sequencing cycle per cluster. Standard read lengths kits available on the Solexa GA are currently 18, 26, and 36bp. A flow cell is broken up into eight lanes; each capable of running a unique sample and generating 150-250Mbp of output or 4-7 million reads per 36bp sequencing run.
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High-throughput Microarray Processing

The IGSB HGAC performs tiling and expression microarray hybridizations using the Tecan HS 4800 PRO Hybridization Station and the Agilent manual SureHyb protocol. Our facility has the current capacity to process 12 slides simultaneously on Tecan and 48 slides simultaneously following Agilent's SureHyb protocol. To date we have processed Agilent arrays in a high-throughput format following Agilent's Gene Expression or ChIP-seq protocols. We have two scanners, a single slide Axon 4000B 5um scanner and a C-version Agilent Autoloader scanner capable of scanning up to 48-slides at a maximum resolution of 2um.
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