Cellular Screening Center (CSC) Core
The Cellular Screening Center (CSC) provides high throughput cellular screening for models of human disease. Both genetic and chemical screenings are performed, which lead to the identification of genes and potentially therapeutically relevant compounds.
Support from the Chicago Biomedical Consortium (CBC) and the University of Chicago Biological Sciences Division enabled the creation of the CSC. The Core facilitates drug discovery by defining complex biology underlying disease. Automation is used for all aspects of the screening process. CSC facilities are available for investigators on a fee-for-service basis.
CSC is located in a certified Class 10,000 clean room (anteroom), a certified Class 1,000 room (cell culture room), and a certified Class 100 (automation area) in the Gordon Center for Integrative Sciences. Resources include one robotic arm (Thermo F3), eight automated liquid handling systems (Matrix PlateMatePlus, three Thermo MultiDrop Combis, one PerkinElmer EP3, one Tecan EVO 100, one Tecan EVO200, one Biotek ELx405UCWVS), one Molecular Devices GT Analyst multimodal screening system, one Molecular Devices ImageXpress automated ﬂuorescence microscope with high-speed autofocus, one TTP Labtech Acumen eX3 fluorescence microplate cytometer, two biosafety cabinets, waterbaths, centrifuges and incubators.
Through the use of automation and robotics, the CSC can screen more than 250,000 fluorescent or luminescent reactions for cellular or enzymatic activities daily and test gene function via RNAi screening of the entire genome three times in a single day. CSC operations are managed by Technical Director, Sam Bettis.
Please direct questions and comments to Sam Bettis, Technical Director, CSC.
Cellular Screening Center (CSC)
The University of Chicago
Gordon Center for Integrative Sciences
929 E. 57th St.
Chicago, IL 60637